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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circularly polarized luminescence. As drawn in Fig - https://urlscan.io/result/3823bc3a-74b6-4d0f-8f09-522e983b4d26/. 9, the response may also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As revealed in Fig. 9, this could be because the H4B radical serve as an electron sink in a later point in the reaction, so as to permit NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo check out this site intermediate that reacts with NOHA.